Gga3 Knockout cell line(PC-12)

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location： Home > Products > KO Cell Lines > Gga3 Knockout cell line(PC-12)

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Gga3 Knockout cell line(PC-12) Order

Gga3 Knockout cell line(PC-12)
Catalog#
YKO-R056
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Gga3 Knockout cell line(PC-12)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Gga3 Knockout cell line (PC-12)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Rat
Gene Name
Gga3
Gene ID
360658
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Gga3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC-12 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Gga3 gene knockout Gga3 Knockout cell line (PC-12) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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